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Nitrofurantoin(AHD) ELISA Test Kit 

  Category : Biotech->Biotech Supplies->blood glucose meters

     
Nitrofurantoin(AHD) ELISA Test Kit
       

  Product Specification
 
  Model No:      RN24S
  Country  US
  Materials  REAGEN

 

    Product Detail
  Product Description
REAGEN Nitrofurantoin (AHD) ELISA Test Kit provides a competitive enzyme immunoassay for the quantitative analysis of nitrofurantoin in feed, fish, shrimp, meat (beef, chicken and pork), eggs, honey, milk, serum and urine.
The unique features of the kit are:
Rapid (4 hours), high recovery ( and cost-effective extraction methods for various samples.
High sensitivity (0.025 ng/g or ppb) and low detection limit (0.05 ng/g or ppb) for various samples.
A quick ELISA assay (less than 1.5 hours regardless of number of samples).
High reproducibility.

Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The AHD antibody has been coated in the plate wells. During the analysis, sample is added along with the AHD-horseradish peroxidase (AHD-HRP) conjugate. If the AHD residue is present in the sample, it will compete for the AHD antibody, thereby preventing the AHD-HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the AHD residue concentration in the sample.

Kit Contents, Storage and Shelf Life
REAGEN Nitrofurantoin (AHD) ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8C*. The shelf life is 12 months when the kit is properly stored.

Kit Contents Amount Storage
AHD Antibody-Coated Plate 1 x 96-well plate (8 wells x 12 strips) 2-8C
AHD Standards:
Negative control (white cap tube)
0.025 ng/mL (yellow cap tube)
0.1 ng/mL (orange cap tube)
0.25 ng/mL (pink cap tube)
0.75 ng/mL (purple cap tube)
2.5 ng/mL (blue cap tube)
10 ng/mL (spiking, optional, red cap tube)
1.8 mL
1.8 mL
1.8 mL
1.8 mL
1.8 mL
1.8 mL
1.8 mL 2-8C
AHD- HRP Conjugate 6 mL 2-8C *
10X Sample Extraction Buffer ** 25 mL 2-8C
20X Wash Solution ** 28 mL 2-8C
Stop Buffer ** 20 mL 2-8C
TMB Substrate ** 12 mL 2-8C
50 mM 2-Nitrobenzaldehyde ** 1.8 mL 2-8C

* If you are not planning to use the kit for over 1 month, store AHD- HRP Conjugate at -20C or in a freezer.

Sensitivity

Sample Type Detection Limit (ng/g or ppb)
Feed 0.1
Fish/Shrimp/Meat 0.05
Egg/Egg Powder 0.05
Honey 0.05
Milk 0.05
Serum 0.05
Urine 0.05

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